Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes.
Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated.
The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to programmed cell death.
One of the major targets for caspase-6 is the membrane associated protein lamin A.
The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15kD). Does not recognize other caspases. Species Crossreactivity: human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6.
