Caspase 3 is one of the key executioners of apoptosis.
It is responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP).
Activation of caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits.
Cleavage of caspase 3 requires aspartic acid at the P1 position
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:800Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry (Paraffin): 1:400 Requires HIER using sodium citrate buffer pH6.0 prior to staining
Immunohistochemistry (Frozen): 1:400 fixed in 3% Formaldehyde
Immunofluorescence (IC): 1:400Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes cleaved human Caspase 3 (Asp175). Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Detects non-specific caspase sunstrates by Western Blot.Species Crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：FC IC IF IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. Species Sequence Homology: porcine, bovine and canine
