Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in the K562 (human chronic myelogenous leukemia) cell line.
The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution.
c-Abl protein is distributed in both the nucleus and the cytoplasm of cells.
In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22.
The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.
It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response (1-3).
c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluoroescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Recognizes bands at 135kD (c-Abl) (210kD (Bcr-Abl))
Immunoprecipitation: 1:100Immunofluorescence: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of Abl proteins. Species Crossreactivity: Human, mouse, rat and drosophila.
Isotype：IgG
Calc Applications Abbrev：E IF IP WB
Calc Crossreactivity：Dr Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminus of human c-Abl.
