Bcl-xL (28kD) is part of the Bcl-2 family, which are important regulators of apoptosis.
Bcl-X has two isoforms, Bcl-xL (long) a 241 amino acid protein and shares significant homology with Bcl-2.
Bcl-xS (short) a 178 amino acid protein lacking a 63 amino acid domain near the transmembrane region that is well conserved among members of the Bcl-2 family.
Like Bcl-2, Bcl-xL has the ability to inhibit apoptosis and is widely studied for its role in tumor generation.
In a recent study, analysis of Hodgkin’s disease showed that 71% of cases (114/160) showed immunohistochemical reactivity with Bcl-xL in Reed-Sternberg cells.
In some cases, Bcl-xL reactivity was present in Reed-Sternberg cells that were negative for CD15 and CD30.
Atypical hyperplasias (0/28) were negative for Bcl-xL
Bcl-xL prevents apoptosis through two different mechanisms: by heterodimerization with an apoptotic protein to inhibit its apoptotic effect (1,2), and by its direct pore-forming effect on the outermembrane of mitochondria to help maintain a normal membrane state under stress conditions (3).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes total levels of endogenous human Bcl-xL protein at ~30kD. Does not crossreact with other Bcl-2 family members. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.
