Bax is a key component for cellular induced apoptosis through mitochondrial stress (1).
Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2).
By interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane’s permeability and leads to the release of cytochrome c from mitochondria, which activates caspase-9 and initiates the caspase activation pathway for apoptosis (3,4).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Bax protein at ~20kD. Does not crossreact with other Bcl-2 family members. Species Crossreactivity: monkey, mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminal residues of human Bax.
