Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-XL, resulting in cell death (1,2).
Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2).
Phosphorylation at these sites results in the binding of Bad to 14–3–3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-XL (2).
Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136 (3,5).
Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK (6,7) and mitochondria-anchored PKA (8).
Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-XL because of its position in the BH3 domain (9–11).
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:200Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For max imum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites, or does it detect related family members. Species Crossreactivity: Human, mousse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：E FC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser112 of mouse Bad. MW: 23kD
