It plays a critical role in controlling the balance between survival and apoptosis (1-3).
This protein kinase is activated by insulin and various growth and survival factors.
it functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3).
Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4).
It is also activated by phosphorylation within the C-terminus at Ser473.
Akt functions to promote cell survival by inhibiting apoptosis by means of its ability to phosphorylate and inactivate several targets, including Bad (5), Forkhead transcription factors (6) and caspase-9.
PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (7).
LY294002 is a specific PI3 kinase inhibitor (8).
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of Akt only when phosphorylated at Thr308. Does not detect Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase. Species Crossreactivity: Human and rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt. Species sequence homology: chicken, xenopus and bovine (100%)
