The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias.
Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF- 16, which has been shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt.
There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319.
Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle.
Phosphorylation regulates FKHR nuclear translocation and target gene transcription.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human Fox04 only when phosphorylated at serine 193. May detect phospho-Fox01. Species Crossreactivity: hamster
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hm Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser193 of human AFX
