4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation.
Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.
The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1.
4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70.
Applications:Suitable for use in Western Blot, and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.)Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Molecular Weight: 15-20kD
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species Cross-Reactivity: human, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
