4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation.
Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.
The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1.
4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70.
Applications:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of mouse 4E-BP1 only when phosphorylated at Ser65. Has also been shown to recognize 4E-BP1 when phosphorylated at serine 101. Species Crossreactivity: human, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
