84-1035-63　Migration Inhibitory Factor (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF) (Biotin) 50ug　M3895-07B

特徴

• MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of macrophages.
• Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system.
• MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids.
• The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production.
• MIF plays a critical role in the host control of imflammation and immunity.
• Applications:Suitable for use in Flow Cytometry, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 2.5ug/10e6 cells.
• HL-60 human acute promyelocytic leukemia cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin
• Western Blot: 0.1ug/ml.
• Recombinant human MIF
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• .
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.