c-myc is involved in the control of cell proliferation and differentiation.
Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (k light chain); or 22 (l light chain) is described in Burkitt’s lymphoma and other B-cell lympho-proliferative conditions.
An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA: 1:250-1:500Western Blot: 1:500-1:1000Immunohistochemistry: 1:100-1:250Immunocytochemistry: 1:250Immunoprecipitation: 1:250Optimal dilutions to be determined by the researcher.
Quality Control:Antibodies were analyzed using ELISA and western blots using an N-terminal FLAG-tagged protein.
Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies, respectively.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied a sterile-filtered liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human c-myc. Recognizes the same epitope as recognized by the 9E10 mouse monoclonal antibody.
Isotype：IgY
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to EQKLISEEDL from human c-myc, conjugated to KLH
