A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene.
p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53.
Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Reacts with a 17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-a and PUMA-b forms of the protein. Immunoprecipitates both forms of the protein. Amino acid identities to mouse homolog is 15/17 (88%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-a and PUMA-b forms of the protein.
