CD100, also known as leukocyte semaphorin), is a homodimeric protein expressed on majority of hemopoietic cells ( B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells.
CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and serin kinase.
It plays a role in homotypic cell adhesion and in T cell activation.
It expression is increased after PHA-activation.
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development.
CD100 is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro.
CD100 modifies CD40-CD40L B-cell signaling by augmenting B-cell aggregation and survival and down-regulating CD23 expression.
CD72 is a lymphocyte receptor for the Class IV semaphorin CD100, which is involved in regulating B cell signaling.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:250-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide before the addition of 40% glycerol.
Specificity：Reacts with residues 147-158 [GKNEDGKGRCPF] of the human CD100 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, aa147-158 [GKNEDGKGRCPF] of the human CD100 protein.
