Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways.
All known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, and skeletal muscle but are very poorly expressed or absent in these tissues in the adult, implying a possible role for the caspase-10 family in fetal development.
Caspase-10 belongs to DED (death effector domain) containing caspases and functions as an initiator caspase in Fas/TNF induced apoptosis (1).
Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d.
They have the same prodomain but different mature large and small subdomains (2–4).
Upon death ligand–receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1–4).
This complex processes procaspase-10 into a large active subunit and a small subunit.
Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death (3–6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot 1:250-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide before the addition of 40% glycerol.
Specificity：Reacts with the N terminus of all isoforms of human Caspase-10.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：N-terminus of all isoforms of human Caspase-10
