High pH heat induced antigen retrieval is recommended
Neutralization: 0.08-0.12ug/ml yields one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (10ng/ml)ELISA: 0.5-2ug/ml using 100ul/well.
Allows the detection of 2000-4000pg/well of recombinant mouse VEGF
Western Blot: 0.1-0.2ug/ml.
The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes mouse VEGF.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse VEGF expressed in E. coli.
