The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes.
A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2).
Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway.
Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3–6).
Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: Mouse heart tissue lysate
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes a 25kD band. Species Crossreactivity: Human and rat.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Raised against a peptide (SDEGADQEEEAYLRED) corresponding to amino acids 222 to 237 of murine Smac/DIABLO (2).
