The p53 tumor-suppressor gene integrates numerous signals that control cell life and death.
Several novel molecules involved in p53 pathway, including CHK2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered.
The transcriptional activity of p53 is modulated by protein stability and acetylation.
PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex (6-8).
PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53.
Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6).
PID/MTA2 is ubiquitously expressed in human tissues (8).
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: HeLa cell lysate
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes a 75kD band. No crossreactivity with MTA1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Raised against a synthetic peptide (PAPSHPASTNEPIVLED) corresponding to amino acids 652 to 668 of human PID/MTA2 (6), which differ from the mouse sequence by one amino acid (7).
