The p53 tumor-suppressor gene integrates numerous signals that control cell life and death.
Several novel molecules involved in p53 network, including CHK2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6), MTBP (7) and PERP (8), were identified and their genes were cloned recently.
PERP, also termed PIGPC1 and THW, is a plasma membrane protein (8-10).
p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis (8).
The expression of PERP causes cell death.
PERP is a mediator of p53 induced apoptosis.
PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family (8).
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: A431 cell lysate
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes a 21kD band.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised against a synthetic peptide (EDDLLGNAKPRYFYTSA) corresponding to amino acids 175-193 of human PERP, which differ from the mouse sequence by three amino acids (7-9).
