Apoptosis is related to many diseases and development.
The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes.
A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3).
PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.
The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1).
PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53.
PUMA may be a direct mediator of p53-induced apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/ml.
Optimal dilutions to be determined by the researcher.
Positive Control:K562 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
Specificity：Recognizes a band at ~23kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised with a synthetic peptide (ARARQEGSSPEPVEG) corresponding to amino acids 2 to 16 of human PUMA-a (1). This sequence is identical between human and mouse PUMA.
