84-1032-57　NFkB Activating Kinase, CT (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1) 100ug　N2301-40

特徴

• Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
• NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli.
• NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
• Phosphorylation of I-kB by IkB kinase (IKK) complex leads to degradation of I-kB and activation of NF-kB.
• The IKK complex contains IKKa, IKKb, and IKKg.
• A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kB-activating kinase), and T2K (1-3).
• NAK/TBK1 activates IKKb through direct phosphorylation.
• NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors (2).
• NAK/TBK1 functions upstream of NIK and the IKK complex (1,2).
• NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).
• Applications: Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
• Positive Control: MOLT4 whole cell lysate
• Storage and Stability: May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.