Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli.
NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKa, IKKb, and IKKg.
A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i (1,2).
IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1 (1).
IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS (2).
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: Jurkat whole cell lysate
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes an 80kD band. No crossreactivity with IKKa, IKKb or IKKg.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised against a peptide (NRIIERLNRVPAPPDV) corresponding to amino acids 701 to 716 of human IKKe/IKK-i (1,2).
