84-1032-52　IAP-Like Protein 2 (ILP 2, Inhibitor of Apoptosis Protein) 100ug　I0400

特徴

• Apoptosis, or programmed cell death, is related to many diseases, such as cancer.
• Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins.
• IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases.
• A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1).
• ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1).
• ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1.
• ILP-2 interacts with the processed caspase-9.
• These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
• Applications: Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution: Western Blot: 1-2ug/ml; Optimal dilutions to be determined by the researcher.
• Positive Control: Human HepG2 or MOLT4 cell lysate
• Storage and Stability: May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.