84-1032-50　DAP Kinase 2 (DAK2, DAP Kinase Related Protein 1, DAPK2, Death Associated Protein Kinase 2) 100ug　D1052

特徴

• Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases.
• Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis.
• DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain.
• Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation.
• DAPK mediates IFNg induced apoptosis.
• A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2).
• Ectopicly expressed DAPK2 induced apoptosis in various types of cells(1,2).
• DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities.
• The messenger RNA of DAPK2 is expressed in multiple human tissues (1).
• Applications: Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
• Positive Control: A431 whole cell lysate
• Storage and Stability: May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.