Three distinct signaling pathways lead to programmed cell death (apoptosis).
The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved.
The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2).
Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion.
Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis.
Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer’s disease including g-secretase/presenilin and b-amyloid precursor protein (APP).
Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-b.
Caspase-12 is ubiquitously expressed in mouse tissues (1).
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 10ug/mlWestern Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Murine spleen tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes mouse Caspase-12. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (AARRTHERDPIYKIKG) corresponding to amino acids 2-17 of murine Caspase 12.
