Apoptosis is related to many diseases and development.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.
Bcl-2 homology 3 (BH3) domain is a potent death domain.
BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family.
A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1).
The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis.
In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell’s actin cytoskeleton.
Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2).
Bmf is constitutively expressed in many tissues (1,2).
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 2ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: HepG2 or 293 cell lysate
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes a band at ~25kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised with a synthetic peptide (ALNGEENRNGAGPR) corresponding to amino acids 171 to 184 of human Bmf (1).
