Apoptosis is related to many diseases and development.
Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis.
A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-b signaling pathway (1).
ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene (1-4) is located to mitochondria and translocates to the nucleus when apoptosis occurs.
ARTS is expressed in many tissues.
It enhances cell death induced by TGF-b and, to a lesser extent, by other apoptotic agents, such as TNF-a and Fas ligand.
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution: Western Blot: 0.5-2ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control: Human lung, kidney and spleen tissue lysates
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes a band at 32kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised against a synthetic peptide (KRFLEDTTDDGE) corresponding to amino acids 3 to 14 of human ARTS (1).
