Applications:Suitable for use in Electron Microscopy, Western Blot, Light Microscopy.
Other applications have not been tested.
Recommended Dilutions:Electron Microscopy: 1:10-1:20Western Blot: 1:10-1:20Light Microscopy: 1:10-1:20Note: BSA and dry milk may contain IgG.
Secondary antibodies directed against bovine, goat, equine or sheep react with bovine IgG.
Use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer.
For blocking, normal serum (5%) from the host species of the labeled secondary is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Gold conjugates are light sensitive.
仕様
Size：300ul
Host：donkey
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M sodium borate-sodium phosphate, 0.15M sodium chloride, pH 8.5, 15mg/ml BSA (Protease and IgG free). 0.05% sodium azide. Labeled with 18nm Colloidal Gold. OD(520) = 2.0.
Specificity：Recognizes whole molecule sheep IgG. Reacts with the light chains of other sheep immunoglobulins. X-Adsorbed and exhibits minimal crossreactivity to chicken, guinea pig, Syrian hamster, equine, human, mouse, rabbit and rat serum proteins.
