Applications:Suitable for use in Electron Microscopy, Western Blot and Light Microscopy.
Other applications have not been tested.
Recommended Dilutions:Electron Microscopy: 1:20-1:40Western Blot: 1:20-1:40 To block, use 5% normal serum from host species to avoid high background due to IgG in BSA and milk.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:300ul
Host:donkey
Source Antibody:goat
Grade:Affinity Purified
Purity:Purified by Immunoaffinity chromatography.
Form:Supplied as a liquid in 0.01M sodium borate-sodium phosphate, 0.15M sodium chloride, pH 8.5, 15mg/ml BSA (IgG and Protease free), 0.05% sodium azide. Labeled with 12nm colloidal gold. OD(520): 2.0.
Specificity:Recognizes goat IgG, H&L. Reacts with the light chains of other goat IgGs. X-Adsorbed against and exhibits minimal crossreactivity to chicken, guinea pig, Syrian hamster, equine, human, mouse, rabbit and rat serum proteins.