Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts.
It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2.
TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections.
TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Activity:≥1x10e5 neutralizing units/mg protein.
Neutralizing capacity has been determined by a cytotoxicity assay in which one unit of TNF-a is assumed to correspond to 50pg of TNF-a protein.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by ammonium sulfate precipitation, Protein A affinity chromatography and immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 125mM trehalose. Reconstitute with 500ul sterile ddH2O.
Specificity：Neutralizes both natural and recombinant mouse TNF-a. Shows a high degree of cross-neutralizing activity with rat TNF-a.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to mouse TNF-a produced in E. coli
