Poly-(ADP-Ribose) Polymerase (PARP) is a 116kD nuclear protein which is strongly activated by binding to DNA strand breaks.
PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation.
During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85kD and a 25kD fragment.
PARP cleavage is considered to be one of the classical characteristics of apoptosis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Recommended Positive Control:HeLa and jurkat cells treated with staurosporine.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Specifically recognizes the 85kD fragment of human cleaved PARP and can be used as marker for detecting apoptotic cells.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to N-terminus of cleavage site (214/215) of human PARP. Species Sequence Homology: hamster, mouse and rat, 86%
