84-1031-76　［取扱停止］FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) 500ug　F0019-58R

特徴

• FAK is a widely expressed non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling.
• Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues.
• Increased FAK tyrosine phophorylation occurs upon integrin engagement with fibronectin.
• Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle.
• In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis.
• FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
• FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase.
• The epitope is localized in the C-terminus (amino acid residues 853-1052).
• Applications:Suitable for use in Immunofluorescence, Western Blotting, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:10-1:50Western Blotting: 5.0 _g/mL with an incubation for 2 hours at RTImmunoprecipitation: 10.0 _g/mg of protein lysate
• Optimal dilutions to be determined by the researcher.
• Recommended Positive Control:HUVEC, NIH3T3 cells, endothelial cells, or kidney tissue.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.