Endothelial Cell Differentiation Gene-1, or EDG-1, encodes a 44kD protein belonging to a family of G-Protein Coupled Receptors (GPCR’s), which bind lysophospholipids.
To date, 8 members of the EDG receptor family have been identified.
These receptors are suggested to have a role in modulation of growth-related effects including cell proliferation and differentiation as well as protective, anti-apoptotic effects.
EDG receptor expression is developmentally regulated and the expression of the 8 members of this family of proteins appears to be tissue specific.
EDG receptors couple to G-proteins to signal through Ras and MAPK pathways and other protein tyrosine kinase pathways.
EDG-1 is also known as S1P1.
The ligand for EDG-1 is sphingosine-1-phosphate.
EDG-1 is expressed predominantly by endothelial cells, as wells as epithelial, neuronal, and cardiovascular cells, but is completely absent from T-lymphocytes.
EDG-1 appears to be important in PDGF-mediated chemotaxis with embryonic fibroblasts.
EDG-1 plays a major role in neuronal function, development and pathology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 5-10ug/mLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.4, 0.08% sodium azide.
Specificity：Recognizes human EDG-1 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal region of the human EDG-1 protein.
