Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 and 43aa peptides (beta amyloid 1-40, 1-42, and 1-43).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
The 140aa a-synucleins is identical with non- Ab component (NACP) of AD.
The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein.
Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold.
Synucleins are primarily expressed in the brain.
At least 3 forms: two large (140aa SYN-1 and 149aa SYN-2) and a small form (SYN-3, 42aa) are produced by alternative splicing.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide is not suitable for use in Western Blot due to its low MW (<3kD)
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well).
Optimal dilutions to be determined by the researcher.
Control Peptide: A2275-75F
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human b-amyloid. Demonstrates a high degree of homology with human and murine b-amyloid (1-40) and b-amyloid (1-42). Weak crossreactivity is observed with b-amyloid (1-28) and b-amyloid (12-28). No crossreaction is observed with CGRP. Species crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo
Immunogen：Human beta Amyloid 1-40 synthetic C-terminal 7 aa peptide, conjugated to KLH.
