84-1031-68　Vesicular Monoamine Transporter 1, CT (VMAT1) 50ug　V2121-88

特徴

• VMAT1 has been localized in adrenal chromaffin cells, endocrine and paracrine cells associated with the intestine, stomach and sympathetic nervous system (2,3).
• VMAT1 has also been found in endosomes of transfected CHO cells and in LDCVs of rat PC12 cells (2).
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-10ug/ml (Chemiluminescence technique).
• Detects a major band at ~55kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells.
• Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors.
• Immunohistochemistry: 1-20ug/ml on paraformaldehyde fixed sections.
• ELISA: 0.5-1.0ug/ml (1ug/ml VMAT1 peptide/well) Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.