Vasopressin (AVP, Arginine-8-Vasopressin), the antidiuretic hormone is cyclic nonapeptide invovled in the homeostasis of body fluid osmolality, blood volume, vascular tone, and blood pressure.
Specific actions of AVP include inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenesis, and modulation of ACTH release from pituitary.
AVP belongs to the family of vasocative and mitogenic peptide invovled in normal and pathological cell growth and differentiation.
AVP exerts its action through binding to specific membrane receptors coupled to distinct second messengers.
There are 3 types of AVP receptors: V1a, V1b, and V2 subtypes.
The V2 receptor stimulates adenyl cyclase and protein Kinase A, V1 activate phospholipase A2.C, and D, resulting into production of IP3 and DAG, the mobilization of intracellular calcium, the influx of extracellular calcium, the activation of protein Kinase C, and protein phosphorylation.
The V1a receptors mediate vasoconstriction and hepatic gluconeogenesis platelet aggregation, coagulation factor release.
V1a receptors are found in vascular smooth muscle, hepatocytes, blood platelets, lymphocytes and monocyte, type II pneumocytes, adrenal cortex, brain, reproductive organs, retinal epithelium, renal mesangial cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
ELISA: 0.5-1ug/ml using 1ug/ml control peptide/well
Immunohistochemistry: 2-10ug in paraformaldehyde fixed sections of tissuesOptimal dilutions to be determined by the researcher.
Recommended Control Peptide:V2120-20A: Vasopressin (Arg) Receptor V1b, Rat, Control Peptide (AVR-V1b)
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat Arginine Vasopressin (Arg) Receptor V1b (AVR-V1b). No significant sequence homology with AVR-V1a or AVR-V2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide correspinding to 18aa near the N-terminal, extracellular domain of rat AVR-V1b, conjugated to KLH. Species Sequence Homology: human, mouse 88%.
