Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons.
VR1 protein is a heat-gated cation channel.
VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0).
Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains.
VR1 expression is limited to small to medium diameter primary sensory neurons.
Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins; rat 761aa, human 764aa) have been cloned and characterized.
There is ~49% identity between VRL-1 and VR1.
In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat.
Instead, it is activated by high temperatures with a threshold of ~52°C.
Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen and intestine) that are unlikely to encounter temperatures of >50°C.
It is possible that VRL-1 is also activated by stimuli other than heat.
Applications:Suitable for use in Western Blot and ELISA Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
Recombinant VR1 is ~90kDELISA: 1:10,000-1:50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: V2075-05A
Reconstitution: Reconstitute with 100ul of sterile dH2O.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and store at -20°C.
Do not freeze.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, serum, 0.09% sodium azide.
Specificity：Recognizes Vanilloid receptor like protein 1 (VRL-1). No significant sequence homology is found with VR1 or other proteins. Species crossreactivity: 100% conserved in mouse VRL-1 and growth factor regulated calcium channel. Only 52% homology with human VRL-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide (18aa) corresponding to the C-terminus of the rat VRL-1 protein.
