84-1031-55　Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase) 100ul　T9237-13

特徴

• Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons.
• It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine.
• TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons.
• The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation.
• At least four isoforms of human TH have been identified which result from alternative splicing.
• Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:1000 on 10ug of PC12 lysates
• Immunohistochemistry: 1:100-1:1000 at 4°C, 1-3 days.
• Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
• Reported not suitable for use on paraffin embedded, human tissue
• Immunofluorescence: 1:100-1:1000; Fixed with 4% PFA overnight at 4°C
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Recommended Positive Control:Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands
• Negative Control:Liver
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.