TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling.
The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth.
TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception).
Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (4).
The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (2).
TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (3).
Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (4).
TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (2).
The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (3) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2500-1:25,000 using akaline phosphataseImmunohistochemistry: 1:1000-1:5000 using PFA (DAB detection)
Immunocytochemistry: 1:1000-5000 (DAB detection)
Optimal dilutions to be determined by the researcher.
Positive Control:Fetal brain/spinal cord tissue.
Human neuroblastoma cell line SMS-KCNR or rat PC12 cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes human TUC-4 protein (ULIP1/CRMP4). Reported to label early postmitotic neurons in prenatal rat brain. Not reactive with the proteins TUC-1 (ULIP3/CRMP1), TUC-2 (ULIP2/CRMP2) or TUC-3 (ULIP4/CRMP3). Does not label neural progenitor or radial glia. Species Crossreactivity: Rat, mouse, feline and monkey.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Fe Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to aa499-511 of TUC-4a and aa612-624 of TUC-4b (YDGPVFDLTTTPK)
