Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
It includes the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml using 5-10ug/ml control peptide per well.
Optimal dilution determined by the researcher.
Control Peptide: T9100-12
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Specificity：Recognizes rat Thiazide-Sensitive Na-Cl Transporter (TSC, NCC). The immunogen shows no significant sequence homology with other CCC. Species Crossreactivity: Rat. Species sequence homology: mouse and rabbit: 88% and human 83%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide, 18 aa sequence near the N-terminus of rat TSC (KLH).
