Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin.
It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin.
It is also the first enzyme in the synthesis of melatonin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detected Tryptophan Hydroxylase on 10ug of PC12 lysates
Useful for cell body but not fiber stainingOptimal dilutions to be determined by the researcher.
Positive Control:Brain tissuePC12 lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, should crossreact with other mammalian species.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Ma Rt
Immunogen：Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
