Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Using silver-intensified immunogold or fluorescence.
Samples should be fixed with 0.05%-0.25% glutaraldehyde for optimum detection.
This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative).The minimum glutaraldehyde concentration is 0.00%.
Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Note: Dilute 100x with 1% GSPBT to ensure enough sample
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 100mM phosphate buffer (1% sterile goat serum), 0.05% thimerosal.
Specificity：Recognizes Taurine. Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue crossreactivity (< 1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.
