Synapsins, a group of at least five members (synapsins Ia, Ib, IIa, IIb and IIIa), are abundant brain proteins essential for regulating neurotransmitter release.
All synapsins contain a short N-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I.
Phosphorylation of synapsin N-terminal domain at serine 9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles.
Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals.
The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis.
It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis.
In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation.
The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS.
The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis.
The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200 to label Protein A, 1:1000 dilution for ECL
Immunocytochemistry: 1:500-1:2500 Immunoprecipitation: 5ul will quantitatively immunoprecipitate all synapsin in 200ug of rat brain.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservative added.
Specificity：Recognizes bovine Synapsin I (Mr of 80,000 and 77,000). Immunolabeling is blocked by preadsorption of antibody with Synapsin I. Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Species Crossreactivity: human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synapsin I (mixture of Ia & Ib) purified from bovine brain.
