Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone.
It is a phosphorylated glycoprotein with an MW ~70-80kD.
The protein has been found in osteoblasts and osteocytes.
BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells.
Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers.
Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling.
Applications:Suitable for use in Western Blot, Immunohistochemistry, RIA and ELISA.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: 1:2500 using 5um paraffin embedded sections from human fetal tibia, fetal calvaria and metastatic breast tumors to bone; with decalcification with 10% formic acidOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservatives added.
Specificity：Recognizes human Bone sialoprotein (BSP). Does not crossreact with human osteopontin or osteonectin by RIA or ELISA. Species Crossreactivity: rat
