84-1031-28　［取扱停止］Serotonin (5-Hydroxytryptamine, 5HT) 100ul　S1001-02

特徴

• Applications:Suitable for use in Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: 1:1000-1:2500 by ABC.
• Glutaraldehyde fixation is required.
• Antibody will not react with non-glutaraldehyde treated tissues.
• Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation.
• Dilute only in PBS or TBS for best results.
• For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
• Optimal dilutions to be determined by the researcher.
• Crossreactivity Data:The crossreactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.
• Compound Crossreactivity:5-Hydroxytryptamine-G-PL1 5-Methoxytryptamine-G-PL 1:25Tryptamine-G-PL 1:60 5-Hydroxytryptophan-G-PL 1:175 5-Hydroxytryptamine 1:1150 Tryptophan-G-PL 1:3400 5-Methoxytryptophan-G-PL 1:20,000
• (G): Glutaraldehyde(PL): Poly-lysine
• The antisera was also tested for specificity using the free-floating PAP technique on rat raphe nuclei.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.