Regulatory factor X5 (RFX5) and Class II Transactivator (CIITA) are two transcriptional activators identified in human patients with bare lymphocyte syndrome.
They bind to "X boxes" in class II MHC gene promoters.
X boxes are conserved among class II MHC genes in humans and mice.
The X box sequence is 5'-GTNRCC(N 0-3 )RGYAAC-3' where N is any nucleotide, R is a purine, and Y is a pyrimidine.
Patients with bare lymphocyte syndrome have no class I or class II MHC molecules due to a complete lack of transcription of class I and class II MHC genes.
Both class I and class II MHC genes are flanked by 5’ promoter sequences, which are bound by specific transcription factors.
Consequences of Bare Lymphocyte Syndrome are an absence of cellular and humoral T cell immuneresponses.
Applications: Western blot: 1:1,000 using fibroblast and B-cell lysate.
Immunoprecipitation EIA Supershift assay: 0.5–1.0ul per assay.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified Ig
Form：Supplied as a sterile filtered liquid in 0.01M Sodium Phosphate buffer, pH 7.4 containing 0.15 M Sodium Chloride with 0.01% sodium azide.
Specificity：Human RFX5 aa 320 to 494. By Western blot it reacts with a major band at 75kD. May react with minor bands at 65 and 80kD.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：RFX5 peptide corresponding to the region at amino acids 320 to 494 of human protein conjugated to KLH
