Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus.
A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate.
This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein.
It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein.
Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders.
Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation.
Binding of Ly-pT-Gly-HRP (0.2ug/100ul/well) to an anti-phosphothreonine coated microplate (0.5ug/100ul/well) is inhibited by 95% with the addition of 2-10ug/ml anti-phosphothreonine.
No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1mg/ml.
Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.0, 0.01% sodium azide, 50% glycerol.
Specificity：Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Immunogen：Phosphothreonine conjugated to KLH and phosvitin.
