84-1031-12　Phosphoserine 50ug　P4076

特徴

• Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins.
• Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease.
• Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with antiphosphoserine or antiphosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:500 detects serine phosphorylated proteins on 10ug of NGF PC12 lysates.
• NGF treated PC12 lysates were resolved by electrophoresis, transferred to PVDF membrane and probed wiht P4076.
• Proteins were visualized using a donkey anti-rabbbit secondary antibody (HRP) and a chemiluminescence detection system
• Immunoprecipitation (Tissue extracts): 10-20ug/mg protein.
• Note that this will immunoprecipitate all phosphoserine proteins in the extracts.
• If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5ug/500ul of extracts.
• ELISA (Kinase assay): 1:250-1:500Immunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
• Positive Controls:NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.