84-1031-10　Peripherin (PRPH, Neurofilament 4, NEF4) 100ul　P3345-01

特徴

• Peripherin is a 56-58kD type III intermediate filament protein, expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors.
• It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g.
• Hirschsprung's disease) states.
• Abnormal inclusions containing peripherin have also been reported in ALS.
• During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration.
• Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
• Applications:Suitable for use in Immunohistochemistry, Western Blot and Electron Microscopy.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry (Frozen, paraffin): 1:100-1:200 Fix frozen sections in acetone at -20°C.
• Works on tissue fixed for one hour or less in fresh 4% paraformaldehyde.
• Western Blot: 1:1000 detected Peripherin on 10ug PC12 lysates.
• PC12 lysate was resolved by electrophoresis, transferred to PVDF membrance and probed with P3345-01.
• Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
• Optimal dilutions to be determined by the researcher.
• Control:Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.