Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms.
In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm.
Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported.
The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291aa respectively.
Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned.
Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes.
It has been suggested that mPer regulates neuronal activity in the SCN.
Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.
Clock has been mapped to chromosome 5.
Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa).
Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, and kidney).
Applications:Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry.
Other applications have not been tested
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL.
Immunohistochemistry: 2-20ug/ml
Immunofluorescence: 2-20ug/mlELISA: 1:10,000-1:100,000 using 50-100ng of control peptide (Cat #P3342-20C)/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes mouse Per 2. No significant sequence homology with human Per2, Per1 or Per3.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 23aa near the C-terminal of human PER2, conjugated to KLH. Species Sequence Homology: rat, 77%
